产品信息

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O⁶-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates. The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual (NEB #E6901). The purified SNAP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C.

Mass spectrometry analysis indicates the SNAP-tag purified protein has two species with molecular weights of 19,694 and 19,829 (with a C-terminal DTT moiety attached).

产品类别:

Snap Tag Other Products,

Protein Labeling Products

应用:

SNAP Companion,

Protein Labeling Snap Clip,

Cell Imaging

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  P9312S     -80       SNAP-tag® Purified Protein P9312SVIAL -80 1 x 0.05 ml 50 µM

• 注意事项

  1. Storage: The SNAP-tag Purified Protein may be stored at -20°C for short term use, but should be kept at -80°C for long term storage. SNAP-tag Purified Protein is supplied at a concentration of 50 μM and is supplied in 1X PBS with 1mM DTT. Thaw and aliquot just before use.
  2. Fresh DTT should be added before use each time.

操作说明、说明书 & 用法

• 操作说明

  1. Labeling SNAP-tag Purified Protein In Vitro (P9312)
  2. View the video “Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging” in the Journal of Visualized Experiments (JoVE)

FAQs & 问题解决指南

• FAQs

  1. Cellular Imaging and Analysis FAQs