产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  R0171V     -20       HpaII R0171VVIAL -20 1 x 0.1 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  R0171S     -20       HpaII R0171SVIAL -20 1 x 0.2 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  R0171L     -20       HpaII R0171LVIAL -20 1 x 1 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  R0171M     -20       HpaII R0171MVIAL -20 1 x 0.2 ml 50,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

• 特性和用法

  单位定义

  一个单位是指在 50 µl 的总反应体系中，在 37℃ 下，1 小时内酶切 1 µg λ  DNA 所需的酶量。

  反应条件

  1X rCutSmart™ 缓冲液
  Incubate at 37°C

  1X rCutSmart™ 缓冲液
  50 mM Potassium Acetate
  20 mM Tris-acetate
  10 mM Magnesium Acetate
  100 µg/ml Recombinant Albumin
  (pH 7.9 @ 25°C)

  在不同缓冲液中的活性

  NEBuffer™ r1.1: 100%
  NEBuffer™ r2.1: 50%
  NEBuffer™ r3.1: <10%
  rCutSmart™ Buffer: 100%

  稀释兼容性

  • 稀释液 A

  贮存溶液

  10 mM Tris-HCl
  50 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  200 µg/ml Recombinant Albumin
  50% Glycerol
  pH 7.4 @ 25°C

  热失活

  80°C for 20 minutes

  甲基化敏感性

  dam 甲基化: 不敏感
  dcm 甲基化: 不敏感
  CpG甲基化: 阻断酶切

  同裂酶

  BsiSI
  HapII
  MspI

• 相关产品

  相关产品

  • t1010-monarch-plasmid-miniprep-kit
  • Monarch® DNA 胶回收试剂盒
  • Monarch® PCR & DNA 纯化试剂盒（5 μg）

  单独销售的组分

  • rCutSmart™ 缓冲液

• 注意事项

  1. HpaII 是 MspI 的同裂酶。
  2. 被盐浓度 > 50 mM 的 KCl 抑制。
  3. CpG 甲基化阻断酶切。

操作说明、说明书 & 用法

• 操作说明

  1. Optimizing Restriction Endonuclease Reactions
  2. Restriction Digest Protocol
  3. Double Digest Protocol with Standard Restriction Enzymes

• 使用指南

  • Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  • Activity of Restriction Enzymes in PCR Buffers
  • Cleavage Close to the End of DNA Fragments
  • Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  • Double Digests
  • Heat Inactivation
  • NEBuffer Activity/Performance Chart with Restriction Enzymes
  • Optimizing Restriction Endonuclease Reactions
  • Restriction Endonucleases – Survival in a Reaction
  • Restriction Enzyme Diluent Buffer Compatibility
  • Restriction Enzyme Tips
  • Single Letter Codes
  • Site Preferences
  • Star Activity
  • Traditional Cloning Quick Guide

工具 & 资源

• 选择指南

  • Alphabetized List of Recognition Sequences
  • Compatible Cohesive Ends and Generation of New Restriction Sites
  • Dam-Dcm and CpG Methylation
  • DNA Methylation Table
  • Frequencies of Restriction Sites
  • Isoelectric Points (pI) for Restriction Enzymes
  • Isoschizomers
  • NEB Diluent and Buffer Table
  • Recleavable Filled-in 5′ Overhangs
  • Restriction Enzymes for Epigenetics Selection Chart
  • Time-Saver™ Qualified Enzymes
  • Why Choose Recombinant Enzymes?

• Web 工具

  • Competitor Cross-Reference Tool
  • DNA Sequences and Maps Tool

  • Double Digest Finder
  • Enzyme Finder
  • NEBcutter™ v3.0
  • NEBioCalculator®
  • REBASE®

FAQs & 问题解决指南

• FAQs

  1. Is HpaII inhibited by salt?
  2. Is HpaII affected by methylation?
  3. What is the molecular weight of HpaII?
  4. Does spermidine increase HpaII activity?
  5. Does HpaII cleave ssDNA?
  6. What is the activity of HpaII at 25°C?
  7. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  8. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
  9. Are HpaII slow sites found in common vectors?
  10. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

• 问题解决指南

  • Restriction Enzyme Troubleshooting Guide