m7G(5′)ppp(5′)A RNA Cap Structure Analog |NEB酶试剂 New England Biolabs

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产品信息

Description: The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).

序列

7mG(5′)ppp(5′)A

产品类别:
RNA Capping

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1405S     -20    
        m7G(5′)ppp(5′)A RNA Cap Structure Analog S1405SVIAL -20 1 x 1 µmol Not Applicable
    • S1405L     -20    
        m7G(5′)ppp(5′)A RNA Cap Structure Analog S1405SVIAL -20 5 x 1 µmol Not Applicable

  • 特性和用法

    提供

    Dried Sodium Salt

    建议使用浓度

    10mM

    推荐重悬体积

    100 μl

  • 注意事项

    1. Addition of 100 µl of water gives approximately a 10 mM solution.

  • 参考文献

    1. Shatkin, A.J. (1978). Cell. 9, 645-653.
    2. Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
    3. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
    4. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
    5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
    6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
    7. Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-6363.
    8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

操作说明、说明书 & 用法

  • 操作说明

    1. Capped RNA Synthesis (E2050)

工具 & 资源

  • 选择指南

    • RNA Cap Analog Selection Chart