Monarch® RNA 裂解液 |NEB酶试剂 New England Biolabs

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产品信息

Monarch® RNA 裂解液 |

Monarch® RNA 裂解液是 Monarch 总 RNA 小量提取试剂盒的组分。可用于裂解细胞并释放细胞组分，包括 RNA。

产品类别:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2012L     25    
        Monarch® RNA Lysis Buffer T2012-1 25 2 x 50 ml Not Applicable

  • 相关产品

    相关产品

    • Luna® 通用一步法 RT-qPCR 试剂盒
    • Luna® 通用探针一步法 RT-qPCR 试剂盒
    • e6560-protoscript-ii-first-strand-cdna-synthesis-kit
    • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
    • NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
    • NEBNext® rRNA 去除试剂盒 – 人/小鼠/大鼠（含 RNA 纯化磁珠）
    • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
    • e7490-nebnext-polya-mrna-magnetic-isolation-module
    • m0368-protoscript-ii-reverse-transcriptase
    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • Monarch® RNA 提取离心柱
    • Monarch® DNA/RNA 保护试剂
    • Monarch® gDNA 去除离心柱
    • Monarch® 收集管 II
    • Monarch® 总 RNA 小量提取试剂盒
    • Monarch® RNA 初始洗涤缓冲液
    • t2014-monarch-rna-wash-buffer
    • Monarch® 总 RNA 小量提取酶套装
    • Nuclease-free Water

操作说明、说明书 & 用法

  • 操作说明

    1. Download Quick Protocol Card T2010
    2. Quick Protocol for Total RNA Extraction (NEB #T2010)
    3. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit

  • 使用指南

    • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
    • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
    • Guidelines for Purification of RNA from Cultured Mammalian Cells
    • Guidelines for RNA Purification from Bacteria
    • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
    • Guidelines for RNA Purification from Plants
    • Guidelines for RNA Purification from Tissues 
    • Guidelines for RNA Purification from Whole Blood
    • Guidelines for RNA Purification from Yeast

  • 应用实例

    • Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit

FAQs & 问题解决指南

  • FAQs

    1. Can I lyse my adherent cells directly in their wells when using the Monarch Total RNA Miniprep Kit?
    2. Can extraction of RNA with the Monarch kits be automated?
    3. Is live SARS-CoV-2 virus inactivated during extraction with the Monarch Total RNA Miniprep Kit?

  • 问题解决指南

    • Troubleshooting Guide for Total RNA Extraction & Purification