Monarch® 总 RNA 小量提取酶套装 |NEB酶试剂 New England Biolabs

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产品信息

Monarch® 总 RNA 小量提取酶套装 |

Monarch® 总 RNA 小量提取酶套装包含 Monarch DNase I 和 Monarch 蛋白酶 K 冻干粉，以及相关反应缓冲液和重悬缓冲液。此套装中的酶经过优化，适合搭配 Monarch 总 RNA 小量提取试剂盒使用，不建议作为 NEB 标准 DNase I 和蛋白酶 K 的替代品。

产品类别:
Total RNA Extraction & Purification Products,
RNA Extraction and Purification,
Nucleic Acid Purification Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T2019L     25    
        Monarch® Proteinase K Reaction Buffer T2003-1 25 1 x 4 ml Not Applicable
        Monarch® DNase I Reaction Buffer T2005-1 25 1 x 4 ml Not Applicable
        Monarch® Nuclease-free Water T2006-21 25 1 x 1.5 ml Not Applicable
        Monarch® Proteinase K T2001-1 25 1 x 20 mg 20 mg/ml
        Monarch® Proteinase K Resuspension Buffer T2002-1 25 1 x 1.5 ml Not Applicable
        Monarch® DNase I T2004-1 25 1 x 275 units 1 units/μl

  • 相关产品

    相关产品

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    • Luna® 通用探针一步法 RT-qPCR 试剂盒
    • e6560-protoscript-ii-first-strand-cdna-synthesis-kit
    • NEBNext® Ultra™ II RNA 非链特异性文库制备试剂盒
    • e7760-nebnext-ultra-ii-directional-rna-library-prep-kit-for-illumina
    • e7765-nebnext-ultra-ii-directional-rna-library-prep-with-sample-purification-beads
    • NEBNext® rRNA 去除试剂盒 – 人/小鼠/大鼠（含 RNA 纯化磁珠）
    • e7490-nebnext-polya-mrna-magnetic-isolation-module
    • m0368-protoscript-ii-reverse-transcriptase
    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • Monarch® RNA 提取离心柱
    • Monarch® DNA/RNA 保护试剂
    • Monarch® gDNA 去除离心柱
    • Monarch® 收集管 II
    • Monarch® RNA 裂解液
    • Monarch® RNA 初始洗涤缓冲液
    • t2014-monarch-rna-wash-buffer
    • Monarch® 总 RNA 小量提取试剂盒
    • Nuclease-free Water

操作说明、说明书 & 用法

  • 操作说明

    1. In-tube DNase I Treatment after Purification with the Monarch Total RNA Miniprep Kit (NEB #T2010)
    2. Quick Protocol for Total RNA Extraction (NEB #T2010)

  • 使用指南

    • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
    • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
    • Guidelines for Purification of RNA from Cultured Mammalian Cells
    • Guidelines for RNA Purification from Bacteria
    • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
    • Guidelines for RNA Purification from Plants
    • Guidelines for RNA Purification from Tissues 
    • Guidelines for RNA Purification from Whole Blood
    • Guidelines for RNA Purification from Yeast

FAQs & 问题解决指南

  • FAQs

    1. Can I skip the on-column DNase I treatment step of the Monarch Total RNA Miniprep Kit protocol?
    2. Can I use leftover Proteinase K and DNase I from the Monarch Total RNA Miniprep Kit for other applications?
    3. Can I use NEB Proteinase K Molecular Biology Grade NEB P8107 in conjunction with the Monarch Total RNA Miniprep Kit?
    4. Can I use NEB DNase I (#M0303) with the Monarch Total RNA Miniprep Kit?

  • 问题解决指南

    • Troubleshooting Guide for Total RNA Extraction & Purification