Sce PUS1 |NEB酶试剂 New England Biolabs

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产品信息

Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer.

Sce PUS1 |
产品类别:
RNA Modification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0526S     -20    
        Sce PUS1 M0526SVIAL -20 1 x 0.05 ml 100 pmol/μl
        NEBuffer™ r1.1 B6001SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    反应条件

    1X NEBuffer™ r1.1
    Incubate at 30°C

    1X NEBuffer™ r1.1
    10 mM Bis-Tris-Propane-HCl
    10 mM MgCl2
    100 µg/ml 重组白蛋白
    (pH 7 @ 25°C)

    热失活

    55°C

  • 相关产品

    相关产品

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    • p8107-proteinase-k-molecular-biology-grade
    • T2030 Monarch RNA Cleanup Kit 10 ug
    • T2040 Monarch RNA Cleanup Kit 50 ug
    • T2050 Monarch RNA Cleanup Kit 500 ug
    • 2X RNA 上样染料
    • 核酸消化混合液

  • 参考文献

    1. Arluison, V., Buckle, M., & Grosjean, H. (1999). Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA.  J Mol Bio. 289 (3), 491-502.
    2. Arluison, V., Batelier, G., Riès-Kautt, M., & Grosjean, H. (1999). RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA (Phe). Biochimie. 81 (7), 751-756.
    3. Carlile, T. M., Martinez, N. M., Schaening, C., Su, A., Bell, T. A., Zinshteyn, B., & Gilbert, W. V (2019). mRNA structure determines modification by pseudouridine synthase 1. Nature Chemical Biolog. 15 (10), 966-974.
    4. (2014). Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature. 515 (725), 143-146.
    5. (1998). The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.. 4 (7), 856-869.
    6. Grünberg S, Doyle LA, Wolf EJ, Dai N, Corrêa IR Jr, Yigit E, et al (2023). The structural basis of mRNA recognition and binding by yeast pseudouridine synthase PUS1. 18(11), DOI: e0291267, PLoS ONE.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for conversion of Uridine to Pseudouridine using Sce PUS1 (NEB #M0526)

FAQs & 问题解决指南

  • FAQs

    1. What is the effect of Mg2+ concentration on Sce PUS1 activity?
    2. How can Sce PUS1 activity be improved when low concentrations of RNA are used?
    3. What effect does MnCl2 have on Sce PUS1 activity?
    4. What is the effect of NaCl concentration on Sce PUS1 activity?
    5. Can Sce PUS1 modify more than 1 µg of RNA in one reaction?
    6. Can I scale up or scale down the reaction volume of the Sce PUS1 reaction?
    7. Can I shorten or extend the incubation time of Sce PUS1 with target RNA?
    8. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?