Monarch® RNase A |NEB酶试剂 New England Biolabs

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产品信息

Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types. Monarch RNase A degrades single-stranded RNA at C and U residues. This formulation is purified from cow pancreas, is free of contaminating protease or DNase activity, and contains no residual host DNA. It is used at 56°C in the Monarch Genomic DNA Purification Kit protocol but is active at any temperature between 20°C and 65°C.  It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4.

Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of  any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low; however, an RNase A treatment step is included in each protocol as an option for the user. 

产品类别:
RNases,
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3018L     -20    
        Monarch® RNase A T3018-2 -20 2 x 0.5 ml 20 mg/ml

  • 特性和用法

    贮存溶液

    50 mM Tris-HCl
    50% Glycerol
    pH 7.4

操作说明、说明书 & 用法

  • 操作说明

    1. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    2. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    4. Genomic DNA Purification from Yeast (NEB #T3010)
    5. Genomic DNA Purification from Saliva (NEB #T3010)
    6. Protocol for Genomic DNA Cleanup (NEB #T3010)
    7. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
    8. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
    9. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    10. Quick Protocol for Extraction and Purification of Genomic DNA

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. Can I use a different RNase A with the Monarch® Genomic DNA Purification Kit?
    2. Can I omit the RNase digestion step from the protocol? 
    3. Can I use Monarch® RNase A for applications/workflows other than gDNA purification?
    4. The enzymes in the kit were not stored at -20°C right away. Will they still work?

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)