RNase If |NEB酶试剂 New England Biolabs

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产品信息

Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA in the absence of calcium (2). RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.

产品来源

An E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).

产品类别:
RNases

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0243S     -20    
        RNase If M0243SVIAL -20 1 x 0.1 ml 50,000 units/ml
        NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X
    • M0243L     -20    
        RNase If M0243LVIAL -20 1 x 0.5 ml 50,000 units/ml
        NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 µl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.

    反应条件

    1X NEBuffer™ 3
    Incubate at 37°C

    1X NEBuffer™ 3
    100 mM NaCl
    50 mM Tris-HCl
    10 mM MgCl2
    1 mM DTT
    (pH 7.9 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    100 mM NaCl
    1 mM DTT
    0.5 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    70°C for 20 minutes

  • 优势和特性

    应用特性

    • Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
    • Used in ribonuclease protection assays

  • 相关产品

    单独销售的组分

    • NEBuffer 3

  • 注意事项

    1. RNase If will not degrade DNA. It has a preference for single-stranded RNA over double-stranded RNA.

  • 参考文献

    1. Spahr, P.F. and Hollingworth, B.R. (1961). J. Biol. Chem.. 236, 823-831.
    2. Grünberg S, Coxam B, Chen TH, Dai N, Saleh L, Corrêa IR, Nichols NM, Yigit E. (2021). Nucleic Acids Res.. May 21;49(9), 5265-5277.
    3. Meador, J. III and Kennell, D. (1990). Gene. 95, 1-7.
    4. Meador, J. III, Cannon, B., Cannistraro, V.J. and Kennell, D. (1990). Eur. J. Biochem.. 187, 549-553.

操作说明、说明书 & 用法

  • 操作说明

    1. Cleavage of ssRNA with RNase If (NEB #M0243)