Monarch® gDNA 细胞裂解液 |NEB酶试剂 New England Biolabs

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产品信息

Monarch gDNA 细胞裂解液是 Monarch 基因组 DNA 提取试剂盒（NEB #T3010）的一个组份。提供温和的裂解条件，有助于降低细胞样本的粘度。该裂解液针对速度和效率进行了优化，只需与 Monarch 蛋白酶 K 和 Monarch RNase A 一起裂解温育 5 分钟，即可获得高纯度、高产量的完整基因组 DNA，其峰值片段大小通常 > 60 kb。

产品类别:
Genomic DNA Extraction & Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T3012L     25    
        Monarch® gDNA Cell Lysis Buffer T3012-2 25 1 x 20 ml Not Applicable

操作说明、说明书 & 用法

  • 操作说明

    1. Quick Protocol for Extraction and Purification of Genomic DNA
    2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
    3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
    4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
    5. Protocol for Genomic DNA Cleanup (NEB #T3010)
    6. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
    7. Genomic DNA Purification from Yeast (NEB #T3010)
    8. Genomic DNA Purification from Saliva (NEB #T3010)
    9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
    10. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)

  • 使用指南

    • Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
    • Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
    • Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

FAQs & 问题解决指南

  • FAQs

    1. What is the composition of each buffer provided with the Monarch® Genomic DNA Purification Kit?
    2. What size gDNA can be purified with the Monarch® Genomic DNA Purification Kit?
       
    3. Can I omit the RNase digestion step from the protocol? 

  • 问题解决指南

    • Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)