Monarch® Plasmid Miniprep Columns |NEB酶试剂 New England Biolabs

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产品信息

Monarch Plasmid Miniprep Columns, supplied with the Monarch Plasmid Miniprep Kit, have been custom designed to deliver excellent performance for your plasmid purification. Monarch Columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carry-over contamination. The tapered design of the Miniprep Column enables elution in as little as 30 μl. Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. The columns fit snugly into the collection tubes to enable easy handling. The Monarch Miniprep Columns also feature a convenient tab so that you can label your columns easily without removing them from your tube rack. Monarch columns are suitable for use with centrifugation and vacuum purification protocols, and are supplied with collection tubes.

Monarch Plasmid Miniprep Column Design
Monarch® Plasmid Miniprep Columns |

重点

  • No buffer retention/risk of carryover contamination
  • Low elution volume (≥ 30 μl)
  • Convenient tab for easy labeling
  • Snug fit into collection tubes for easy handling
  • Made with less plastic than conventional columns
产品类别:
Plasmid Purification Products,
Nucleic Acid Purification Products

应用:
Nucleic Acid Purification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • T1017L     25    
        Monarch® Plasmid Miniprep Columns T1017-2 25 2 x 50 columns Not Applicable

  • 特性和用法

    结合容量

    20 µg

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® 质粒重悬缓冲液（B1）
    • Monarch® 质粒裂解液（B2）
    • Monarch® 质粒中和缓冲液（B3）
    • Monarch® 质粒洗涤缓冲液 1
    • Monarch® 质粒洗涤缓冲液 2
    • Monarch® DNA 洗脱缓冲液
    • Monarch® DNA 纯化离心柱（5 μg）
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）
    • Quick-Load® 紫色 1 Kb Plus DNA Ladder
    • 1 kb Plus DNA Ladder

    单独销售的组分

    • Monarch® 质粒重悬缓冲液（B1）
    • Monarch® 质粒裂解液（B2）
    • Monarch® 质粒中和缓冲液（B3）
    • Monarch® 质粒洗涤缓冲液 1
    • Monarch® 质粒洗涤缓冲液 2
    • Monarch® DNA 洗脱缓冲液

操作说明、说明书 & 用法

  • 操作说明

    1. Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010)

  • 使用指南

    • Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
    • Dos and Don’ts of Plasmid Minipreps
    • Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids

  • 应用实例

    • Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit
    • A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

FAQs & 问题解决指南

  • FAQs

    1. Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free?
    2. I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to improve yield and purity?
    3. If I need to clean up more than 5 μg of DNA, is there another higher capacity column alternative to the columns included in the Monarch PCR & DNA Cleanup kit (NEB #T1030)?

  • 问题解决指南

    • Troubleshooting Guide for DNA Cleanup and Plasmid Purification