Next® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing |NEB酶试剂 New England Biolabs

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产品信息

This module contains the following reagents, at volumes designed for use in several protocols alongside Oxford Nanopore Technologies SQK-LSK114:

NEBNext® FFPE DNA Repair Mix (0.048 ml/0.192 ml)
NEBNext® FFPE DNA Repair Buffer (0.084 ml/0.336 ml)
NEBNext® Ultra™ II End Prep Enzyme Mix (0.072 ml/0.288 ml)
NEBNext® Ultra™ II End Prep Reaction Buffer (0.084 ml/0.336 ml)
Quick T4 DNA Ligase (0.240 ml/0.960 ml)
 
The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use of these reagents.

产品类别:
Next Generation Sequencing Library Preparation Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E7180S     -20    
        NEBNext® FFPE DNA Repair Buffer E7181AVIAL -20 1 x 0.084 ml Not Applicable
        NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not Applicable
        NEBNext® Ultra™ II End Prep Reaction Buffer E7183AVIAL -20 1 x 0.084 ml Not Applicable
        NEBNext® Ultra™ II End Prep Enzyme Mix E7184AVIAL -20 1 x 0.072 ml Not Applicable
        Quick T4 DNA Ligase E7185AVIAL -20 1 x 0.24 ml Not Applicable
    • E7180L     -20    
        NEBNext® FFPE DNA Repair Buffer E7181AAVIAL -20 1 x 0.336 ml Not Applicable
        NEBNext® FFPE DNA Repair Mix E7182AAVIAL -20 1 x 0.192 ml Not Applicable
        NEBNext® Ultra™ II End Prep Reaction Buffer E7183AAVIAL -20 1 x 0.336 ml Not Applicable
        NEBNext® Ultra™ II End Prep Enzyme Mix E7184AAVIAL -20 1 x 0.288 ml Not Applicable
        Quick T4 DNA Ligase E7185AAVIAL -20 1 x 0.96 ml Not Applicable

  • 优势和特性

    Features

    Component volumes tailored for use with singleplex SQK-LSK114 ligation sequencing

    Simplified ordering and inventory management

    Compatible with all devices: MinION®, GridION®, PromethION™, Flongle®

    No more waste – no unnecessary buffers or excess reagents

操作说明、说明书 & 用法

  • 操作说明

    1. Usage guidelines for reagents included in the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7180)

FAQs & 问题解决指南

  • FAQs

    1. Which protocol should be followed when using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing?
    2. What type and how much starting material do I need to use when preparing libraries using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing?
    3. Are the reagents supplied in the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing sufficient to construct libraries for Oxford Nanopore technologies devices?
    4. Are the reagents supplied in the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing recommended for native barcoding multiplex sequencing?