Next® 表观遗传建库多样本引物试剂盒 3（Unique 双端 Index 引物） |NEB酶试剂 New England Biolabs

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产品信息

在 PCR 过程中，使用这些独特的双端索引引物对引入索引，这些引物针对 Illumina 测序平台 NEBNext 表观遗传学实验流程进行了优化，包含 NEBNext E5hmC-seq。引物为减少标签跳跃而设计，能标记多个样本。提供 96 个预混的 i5 和 i7 8 碱基索引引物对，包装在一个一次性的密封着可穿刺的铝箔封板膜 96 孔板中，便于一次性使用。

NEBNext 表观遗传建库多样本引物试剂盒 3（Unique 双端 Index 引物）（NEB #E3404S）和 24 次反应的 NEBNext 表观遗传建库多样本引物试剂盒 2B（Unique 双端 Index 引物）（NEB #E3392S）引物兼容，组合使用可以标记多达 120 个样本。

使用 NEBNext 表观遗传建库多样本引物时，请参阅 NEBNext 酶学转化法 5hmC 甲基化建库试剂盒说明书（NEB #E3350）。

产品类别:
NEBNext® Multiplex Oligos (Adaptors & Primers) Products,
Methylome Analysis Products,
Next Generation Sequencing Library Preparation Products

  • 试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E3404S     -20    
        NEBNext® Primers for Epigenetics (Unique Dual Index Set 3) E3404AVIAL -20 1 x 5 μl/well 10 µM

  • 相关产品

    相关产品

    • NEBNext® 酶学转化法 5hmC 甲基化建库试剂盒
    • NEBNext® 表观遗传建库多样本引物试剂盒 2B（Unique 双端 Index 引物）

操作说明、说明书 & 用法

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE3392_E3404

  • 使用指南

    • Forward Strand Workflow Sample Sheet for E3404: NovaSeq 6000 with v1.0 reagent kits, MiniSeq with Rapid reagent kits, MiSeq, HiSeq 2000/2500 (paired-end flow cell), HiSeq 3000/4000 (single-read flow cell)
    • NEB_E3404_UDI_IlluminaLRM
    • Reverse Complement Workflow Sample Sheet for E3404: iSeq 100, MiniSeq with Standard reagent kits, NextSeq Systems, NovaSeq 6000 with v1.5 reagent kits, HiSeq 2000/2500 (single-read flow cell), HiSeq 3000/4000 (paired-end flow cell)

工具 & 资源

  • Web 工具

    • NEBNext Selector

FAQs & 问题解决指南

  • FAQs

    1. Are Sample Sheets available for use with the NEBNext® Primers for Epigenetics?
    2. Are dual-indexed libraries compatible with single end sequencing?
    3. What is provided in the NEBNext® Primers for Epigenetics?
    4. Can the NEBNext® Primers for Epigenetics be used to address “index hopping” with Illumina® patterned flow cells?
    5. How many indices are available with the 24 reaction or 96 reaction size of the NEBNext® Primers for Epigenetics?
    6. Are the NEBNext® Primers for Epigenetics the same as NEBNext® Multiplex Oligos for Illumina®?
    7. Are the NEBNext® Primers for Epigenetics validated for the E5hmC-seq™ workflow?
    8. What is the concentration of the Index Primers in NEBNext® Primers for Epigenetics?
    9. How many reactions worth of primer are in each well for the NEBNext® Primers for Epigenetics?
    10. Can I use the 96-well primer plate supplied with the NEBNext® Primers for Epigenetics to do my PCR reactions?
    11. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext® Primers for Epigenetics on Illumina® sequencing instruments?
    12. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
    13. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?