Lambda Exonuclease |NEB酶试剂 New England Biolabs

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产品信息

重点

Isolated from a recombinant source
Highly processive 5’→3′ exonuclease
Supplied in 10X Reaction Buffer

产品来源

A genetic fusion of the E. coli Lambda Exonuclease gene with the gene encoding maltose binding protein (MBP). Following affinity chromatography, Lambda Exonuclease is cleaved from the fusion construct and purified away from MBP. 

产品类别:
Exonucleases and Non-specific Endonucleases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0262S     -20    
        Lambda Exonuclease M0262SVIAL -20 1 x 0.2 ml 5,000 units/ml
        Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X
    • M0262L     -20    
        Lambda Exonuclease M0262LVIAL -20 1 x 1 ml 5,000 units/ml
        Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [3H]-DNA.

    反应条件

    1X Lambda Exonuclease Reaction Buffer
    Incubate at 37°C

    1X Lambda Exonuclease Reaction Buffer
    67 mM Glycine-KOH
    2.5 mM MgCl2
    50 µg/ml BSA
    (pH 9.4 @ 25°C)

    使用浓度

    5,000 units/ml

    贮存溶液

    25 mM Tris-HCl
    50 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    75°C for 10 minutes

    分子量

    理论上的: 28000 daltons

    比活度

    50,000 units/mg

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

  • 注意事项

    1. Lambda 核酸外切酶在各种缓冲液中的活性如下所示：

      • NEBuffer 1：15%
      • NEBuffer 2：40%
      • NEBuffer 3：10%
      • NEBuffer 4：40%
      • CutSmart：50%
      • 核酸外切酶 I 反应缓冲液：100%
      • Thermopol 反应缓冲液：100%
    2. 5´ 羟基末端的酶切速度比 5´ 磷酸末端慢 20 倍。单链 DNA 的酶切速度比双链 DNA 慢 100 倍。

  • 参考文献

    1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for Lambda Exonuclease (M0262)

  • 使用指南

    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南

    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具

    • Exo Selector

  • 研究摘要

    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs

    1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
    2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    4. Will Lambda Exonuclease work in rCutSmart Buffer?
    5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?