T7 Exonuclease |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

重点

  • Isolated from a recombinant source
  • 5′ -> 3′ exonuclease
  • Supplied with 10X Reaction Buffer

产品来源

Purified from an E. coli strain containing a TYB12 intein fusion.

产品类别:
Exonucleases and Non-specific Endonucleases Products

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0263S     -20    
        T7 Exonuclease M0263SVIAL -20 1 x 0.1 ml 10,000 units/ml
        NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
    • M0263L     -20    
        T7 Exonuclease M0263LVIAL -20 1 x 0.5 ml 10,000 units/ml
        NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H]-DNA.

    反应条件

    1X NEBuffer™ 4
    Incubate at 25°C

    1X NEBuffer™ 4
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    1 mM DTT
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ 1: 30%
    NEBuffer™ 2: 25%
    NEBuffer™ 3: 1%
    NEBuffer™ 4: 100%

    贮存溶液

    10 mM Tris-HCl
    5 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    否

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒（5 μg）

    单独销售的组分

    • NEBuffer 4

  • 参考文献

    1. Kerr, C. and Sadowski, P.D. (1972). J. Biol. Chem. 247, 305-318.
    2. Shinozaki, K. and Okazaki, T. (1978). Nucl. Acids. Res. 5, 4245-4261.

操作说明、说明书 & 用法

  • 操作说明

    1. Protocol for T7 Exonuclease (M0263)

  • 使用指南

    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南

    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具

    • Exo Selector

  • 研究摘要

    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs

    1. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    2. How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)?
    3. What is the activity of T7 Exonuclease in the NEBuffers?
    4. Can T7 Exonuclease be heat inactivated?
    5. What is the best way to generate partials using T7 Exonuclease?
    6. Can DNA be blunted using T7 Exonuclease?
    7. Will T7 Exonuclease work in rCutSmart buffer?
    8. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

  • 实验技巧

    Addition of 6 phosphorothioates to the 5’ end of DNA can block activity up to 90%.