上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
含 UDG 的 WarmStart® LAMP 变色预混液是由 Bst 2.0 WarmStart DNA 聚合酶和 WarmStart® RTx 反转录酶在特殊的低缓冲力反应溶液中优化形成,该溶液包含可视化的 pH 指示剂,使检测 LAMP 和 RT-LAMP 的结果判定更加简单、快捷。预混液中包含的 dUTP 和 UDG 可有效减少反应间的残留污染。
该系统提供了一种快速、清晰可视化的扩增检测方案,其原理为 DNA 聚合酶在 LAMP 扩增反应中产生大量质子,引发 pH 值的下降,从而使反应液颜色从粉红色变为黄色(如需了解 LAMP 概述和引物设计,请观看 NEB 网站相关视频)。该预混液即可用于 LAMP 也可用于 RT-LAMP 反应,仅需加热装置及样品,即可在 15-40 分钟内肉眼判定阳性结果。
- 产品类别:
- Isothermal Amplification & Strand Displacement Products,
- PCR, qPCR & Amplification Technologies Products
- 应用:
- Loop-Mediated Isothermal Amplification,
- Isothermal Amplification,
- DNA Amplification, PCR & qPCR
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产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M1804S -20 WarmStart® Colorimetric LAMP 2X Master Mix with UDG M1804SVIAL -20 1 x 1.32 ml 2 X
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M1804L -20 WarmStart® Colorimetric LAMP 2X Master Mix with UDG M1804SVIAL -20 5 x 1.32 ml 2 X
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优势和特性
应用特性
- LAMP amplification of RNA and DNA targets
- Simple visual indication of amplification for easy detection and analysis
- UDG and dUTP included in master mix for carryover prevention
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相关产品
相关产品
- 等温扩增缓冲液套装
- e1700-warmstart-lamp-kit-dna-rna
- m0380-warmstart-rtx-reverse-transcriptase
操作说明、说明书 & 用法
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操作说明
- WarmStart Colorimetric LAMP 2X Master Mix with UDG Typical LAMP Protocol (NEB #M1804)
工具 & 资源
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选择指南
- DNA Polymerase Selection Chart
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Web 工具
- NEB LAMP Primer Design Tool
FAQs & 问题解决指南
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FAQs
- What is the difference between using Colorimetric LAMP and regular LAMP reactions?
- How long should I incubate the Colorimetric LAMP reaction before checking the results?
- What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
- What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix?
- Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
- How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
- How do I design LAMP Primers?
- The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
- What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
- What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
- Does the WarmStart® Colorimetric LAMP Master Mix with UDG enable carryover prevention/contamination reduction?
- How much sample can be added to the colorimetric LAMP reaction as input?
- Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
- What components are contained in the WarmStart® Colorimetric LAMP Master Mix with UDG and what else I need to perform a LAMP reaction?
- Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
- What is the optimal LAMP/RT-LAMP amplification temperature?
- How fast should I expect a result?
- Can I set up my LAMP/RT-LAMP reactions at room temperature?
- Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
- Is a separate incubation step required for RT-LAMP?
- Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
- Does NEB have a master mix for LAMP or RT-LAMP reactions?
- What is LAMP and RT-LAMP?
- What type of purification is recommended for LAMP primers?