WarmStart® LAMP 变色预混液，含 UDG |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

产品信息

含 UDG 的 WarmStart® LAMP 变色预混液是由 Bst 2.0 WarmStart DNA 聚合酶和 WarmStart® RTx 反转录酶在特殊的低缓冲力反应溶液中优化形成，该溶液包含可视化的 pH 指示剂，使检测 LAMP 和 RT-LAMP 的结果判定更加简单、快捷。预混液中包含的 dUTP 和 UDG 可有效减少反应间的残留污染。 

该系统提供了一种快速、清晰可视化的扩增检测方案，其原理为 DNA 聚合酶在 LAMP 扩增反应中产生大量质子，引发 pH 值的下降，从而使反应液颜色从粉红色变为黄色（如需了解 LAMP 概述和引物设计，请观看 NEB 网站相关视频）。该预混液即可用于 LAMP 也可用于 RT-LAMP 反应，仅需加热装置及样品，即可在 15-40 分钟内肉眼判定阳性结果。

简单可视化的扩增检测

WarmStart® LAMP 变色预混液，含 UDG |

阴性结果显示粉红色，阳性结果显示变为黄色
产品类别:
Isothermal Amplification & Strand Displacement Products,
PCR, qPCR & Amplification Technologies Products

应用:
Loop-Mediated Isothermal Amplification,
Isothermal Amplification,
DNA Amplification, PCR & qPCR

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M1804S     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix with UDG M1804SVIAL -20 1 x 1.32 ml 2 X
    • M1804L     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix with UDG M1804SVIAL -20 5 x 1.32 ml 2 X

  • 优势和特性

    应用特性

    • LAMP amplification of RNA and DNA targets
    • Simple visual indication of amplification for easy detection and analysis
    • UDG and dUTP included in master mix for carryover prevention 
     

  • 相关产品

    相关产品

    • 等温扩增缓冲液套装
    • e1700-warmstart-lamp-kit-dna-rna
    • m0380-warmstart-rtx-reverse-transcriptase

操作说明、说明书 & 用法

  • 操作说明

    1. WarmStart Colorimetric LAMP 2X Master Mix with UDG Typical LAMP Protocol (NEB #M1804)

工具 & 资源

  • 选择指南

    • DNA Polymerase Selection Chart

  • Web 工具

    • NEB LAMP Primer Design Tool

FAQs & 问题解决指南

  • FAQs

    1. What is the difference between using Colorimetric LAMP and regular LAMP reactions?
    2. How long should I incubate the Colorimetric LAMP reaction before checking the results?
    3. What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
    4. What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix? 
    5. Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
    6. How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
    7. How do I design LAMP Primers?
    8. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
    9. What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
    10. What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
    11. Does the WarmStart® Colorimetric LAMP Master Mix with UDG enable carryover prevention/contamination reduction?
    12. How much sample can be added to the colorimetric LAMP reaction as input?
    13. Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
    14. What components are contained in the WarmStart® Colorimetric LAMP Master Mix with UDG and what else I need to perform a LAMP reaction?
    15. Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
    16. What is the optimal LAMP/RT-LAMP amplification temperature?
    17. How fast should I expect a result?
    18. Can I set up my LAMP/RT-LAMP reactions at room temperature?
    19. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
    20. Is a separate incubation step required for RT-LAMP?
    21. Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
    22. Does NEB have a master mix for LAMP or RT-LAMP reactions?
    23. What is LAMP and RT-LAMP?
    24. What type of purification is recommended for LAMP primers?