上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
产品信息
LunaScript 反转录 SuperMix 为经过优化的预混液,可用于第一链 cDNA 合成、扩增子测序,以及两步法 RT-qPCR。该预混液含有具备热稳定特性的 Luna 反转录酶,可在更高温下合成 cDNA。试剂盒还提供小鼠 RNase 抑制剂,以保护模板 RNA 免受降解。LunaScript 反转录 SuperMix 混合液含有随机六聚体和 poly-dT 引物,可均匀覆盖整个 RNA 靶标。此外,混合液还包含蓝色染料,可在两步法 RT-qPCR 起到示踪作用。总 RNA 起始量从高达 1 µg 到低至单拷贝 RNA,均能得到稳定、线性和灵敏的检测结果。
LunaScript 反转录预混液试剂盒(无引物)(NEB #E3025)是经过优化的预混液产品,包含除引物外的第一链 cDNA 合成所需的所有组分,适用于 RT-PCR、RNA-seq 及其它应用。该混合液与随机引物、oligo dT 引物以及基因特异性引物兼容,让 cDNA 合成更灵活。更多信息请点击此处。
与 LunaScript 试剂盒版本(NEB #E3010)相比,此 LunaScript 产品不含无核酶水和 No-RT 对照混合液。更多信息如下:
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b4387c279.jpg)
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b4389d8ca.jpg)
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b438b5cb7.png)
A. 将梯度稀释的 Jurkat 总 RNA(1 μg – 1 pg)反转录为 cDNA,然后通过 qPCR 对 β-actin 靶标进行定量。
B. ERCC(External RNA Controls Consortium)对照 mix 1 RNA 包含 50 – 5×109 拷贝的 ERCC00130(~10 ng – 10 fg),反转录为 cDNA 后通过 qPCR 进行定量。
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b438d3fd0.jpg)
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b438f0328.jpg)
详细了解此“Dots in Boxes”可视化荧光定量评价方法。
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b439117c3.png)
![LunaScript® 反转录 SuperMix 试剂盒 |](http://www.utopbio.com/wp-content/uploads/2024/04/20240418_6620b4392a208.jpg)
详细了解此“Dots in Boxes”可视化荧光定量评价方法。
- 产品类别:
- Luna® qPCR & RT-qPCR Products
- 应用:
- qPCR & RT-qPCR,
- Reverse Transcription (cDNA Synthesis)
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试剂盒组成
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
E3010S -20 LunaScript® RT SuperMix M3010SVIAL -20 1 x 0.1 ml 5 X No-RT Control Mix M3011SVIAL -20 1 x 0.1 ml 5 X Nuclease-free Water B1502AVIAL -20 1 x 1.5 ml Not Applicable
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E3010L -20 LunaScript® RT SuperMix M3010LVIAL -20 1 x 0.4 ml 5 X No-RT Control Mix M3011LVIAL -20 1 x 0.4 ml 5 X Nuclease-free Water B1502AVIAL -20 4 x 1.5 ml Not Applicable
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相关产品
相关产品
- Luna® 通用 qPCR 预混液
- Luna® 通用探针法 qPCR 预混液
- 南极热敏 UDG
- Monarch® 总 RNA 小量提取试剂盒
操作说明、说明书 & 用法
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操作说明
- Protocol for LunaScript® RT SuperMix Kit (E3010)
- Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004)
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说明书
产品说明书包含产品使用的详细信息、产品配方和质控分析。- manualE3010
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应用实例
- Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents
FAQs & 问题解决指南
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FAQs
- Can I use RNA samples purified from different storage conditions such as RNALater in my LunaScript® cDNA synthesis reactions?
- Can I add gene-specific primers in my LunaScript® cDNA synthesis reaction?
- Can I set up my cDNA synthesis reaction at room temperature when using the LunaScript® RT SuperMix?
- Can I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript® RT SuperMix?
- Can I use LunaScript cDNA products directly in qPCR? How much can I use for detection in a qPCR reaction?
- How much RNA template should I use in my LunaScript® cDNA synthesis reactions?
- How should I store LunaScript® cDNA products?
- How should I choose between a one-step RT-qPCR or two-step RT-qPCR approach?
- Should I include a No-Reverse Transcriptase (RT) control reaction when setting up my cDNA synthesis reaction?
- What qPCR reagents do you recommend for detection of cDNA products?
- What temperature should I use for my LunaScript® cDNA synthesis reaction?
- Why do I have low cDNA yields?
- Will the blue dye in the LunaScript® RT SuperMix interfere with detection?
- How do I choose between the first strand cDNA synthesis kits from NEB?
- When using LunaScript cDNA products in downstream PCR (i.e., two-step RT-PCR), what volumes and PCR products can be used?
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Can I use LunaScript RT SuperMix in RNA-seq workflows?
- How do I choose between LunaScript RT SuperMix Kit and ProtoScript first strand cDNA synthesis kits?
- Can I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript® RT Master Mix (Primer-free)? Does longer incubation increase the yield of cDNA?
- How do I choose between Induro Reverse Transcriptase and the different LunaScript and ProtoScript products?
- How do I know whether my template RNA is of good quality?