WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |NEB酶试剂 New England Biolabs

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产品信息

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 

Simple Visualization of Amplification Success

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.
产品类别:
Isothermal Amplification & Strand Displacement Products

应用:
Loop-Mediated Isothermal Amplification,
Isothermal Amplification

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M1800S     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 1 x 1.25 ml 2 X
    • M1800L     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 5 x 1.25 ml 2 X

  • 优势和特性

    应用特性

    • LAMP amplification of RNA and DNA targets
    • Simple visual indication of amplification for easy detection and analysis
    • One-step field and point-of-need LAMP reactions
    • Easy demonstration of LAMP assays with built-in detection

  • 相关产品

    相关产品

    • m0538-bst-20-warmstart-dna-polymerase
    • m0380-warmstart-rtx-reverse-transcriptase
    • 等温扩增缓冲液套装
    • dNTP 混合液
    • e1700-warmstart-lamp-kit-dna-rna
    • m0374-bst-3-0-dna-polymerase
    • M1804 WarmStart Colorimetric LAMP 2X Master Mix with UDG

操作说明、说明书 & 用法

  • 操作说明

    1. WarmStart Colorimetric LAMP 2X Master Mix Typical LAMP Protocol (M1800)

  • 应用实例

    • Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments
    • Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents

工具 & 资源

  • Web 工具

    • NEB LAMP Primer Design Tool

FAQs & 问题解决指南

  • FAQs

    1. What is the difference between using Colorimetric LAMP and regular LAMP reactions?
    2. How long should I incubate the Colorimetric LAMP reaction before checking the results?
    3. What components are contained in the WarmStart® Colorimetric LAMP 2X Master Mix and what else do I need to perform a LAMP reaction?
    4. What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix? 
    5. Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
    6. How do I design LAMP Primers?
    7. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
    8. How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
    9. What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
    10. What components are contained in the WarmStart Colorimetric LAMP Master Mix and what else I need to perform a LAMP reaction?
    11. What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
    12. What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
    13. Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
    14. How much sample can be added to the colorimetric LAMP reaction as input?
    15. Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
    16. Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
    17. What is the optimal LAMP/RT-LAMP amplification temperature?
    18. How fast should I expect a result?
    19. Can I set up my LAMP/RT-LAMP reactions at room temperature?
    20. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
    21. Is a separate incubation step required for RT-LAMP?
    22. Does NEB have a master mix for LAMP or RT-LAMP reactions?
    23. What is LAMP and RT-LAMP?
    24. What type of purification is recommended for LAMP primers?