Anti-M13 pIII Monoclonal Antibody |NEB酶试剂 New England Biolabs

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产品信息

Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes nonreduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage not recommended since the epitope lies in a region not accessible on intact M13 phage virions.

产品类别:
Discontinued (<3 years)

  • 特性和用法

    Antibody Type

    Monoclonal

    Isotype

    Mouse Isotype IgG2a

    Recommended Dilution for Western Blot

    1:1000

  • 优势和特性

    应用特性

    • Recombinant protein domains such as antibody fragments can be fused to M13 coat protein pIII and displayed on phage using phagemid-based systems (1), rather than the phage-based system utilized in the Ph.D. libraries. Anti-M13 pIII Monoclonal Antibody can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis.

  • 注意事项

    1. Store undiluted

操作说明、说明书 & 用法

  • 操作说明

    1. Western Blot (NEB #E8033)