BG-NH2 |NEB酶试剂 New England Biolabs

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产品信息

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-tag substrate is described in this document.

Figure 1: Structure of BG-NH2 (MW 270.3 g/mol) BG-NH2 |
产品类别:
Discontinued (<3 years)

  • 注意事项

    1. BG-NH2 干粉应在 -20℃ 条件下贮存。
    2. BG-NH2 应溶解在无水 N,N-二甲基甲酰胺（DMF）中使用。BG-NH2 可在无水 DMF 中少量溶解。在 37℃ 下加热、涡旋和/或超声处理将有助于溶解。预计会有一些剩余的沉淀物，并应被转移到反应中。随着偶联反应的进行，剩余的固体将继续溶解。选择与最终用途相适应的溶剂体积。在 DMF 中溶解的未使用的 BG-NH2 底物，应在 -20℃ 条件下贮存。

  • 参考文献

    1. Keppler A. et al. (2004). Methods. 32, 437-444.
    2. Keppler A. et al. (2003). Nature Biotechnology. 21, 86-89.

操作说明、说明书 & 用法

  • 操作说明

    1. Reaction Conditions for Chemical Coupling (S9148)
    2. Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)

工具 & 资源

  • 选择指南

    • Building Blocks
    • Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP

FAQs & 问题解决指南

  • FAQs

    1. Cellular Imaging and Analysis FAQs

  • 问题解决指南

    • Labeling with SNAP-tag® Technology Troubleshooting Guide