uffer 3.1 |NEB酶试剂 New England Biolabs

上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。

产品信息

New England Biolabs 为每种限制性内切酶提供了一种采用颜色标记的 10X NEBuffer 反应缓冲液,确保酶保持最佳(100%)活性。我们的大多数酶都会随酶附带四种标准 NEBuffer 缓冲液中的一种。有时,四种标准 NEBuffer 缓冲液均无法满足酶对缓冲液的特定要求,在这种情况下,会随酶提供其专用的 NEBuffer 缓冲液。

产品类别:
Discontinued (<3 years)

  • 特性和用法

    1X 缓冲液组分

    100 mM NaCl
    50 mM Tris-HCl
    10 mM MgCl2
    100 µg/ml BSA
    pH 7.9@25°C

  • 相关产品

    相关产品

    • t1010-monarch-plasmid-miniprep-kit
    • Monarch® DNA 胶回收试剂盒
    • Monarch® PCR & DNA 纯化试剂盒(5 μg)

  • 注意事项

    1. 10X NEBuffer 3.1 的 pH 值范围为 7.8 到 8.0。

工具 & 资源

  • 选择指南

    • NEB Diluent and Buffer Table

FAQs & 问题解决指南

  • FAQs

    1. What is supplied with the NEBuffer 3.1 pack?
    2. You have replaced NEBuffer 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?
    3. Why did you add BSA into all the restriction enzyme reaction buffers?
    4. Why did you remove DTT from your restriction enzyme buffers?
    5. I don’t want to use BSA in my buffer.  What are my options?
    6. Are the alkaline phosphatases active in NEBuffers?