Ph.D.™-12 噬菌体展示肽库试剂盒 |NEB酶试剂 New England Biolabs

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产品信息

Ph.D.-12 噬菌体展示肽库的制备原理是将随机十二肽组合库融合表达于 M13 噬菌体次要外壳蛋白（pIII）（1–6）。展示的肽（12-mer）在 pIII 的 N 端表达，即成熟蛋白的第一残基属于第一个随机位点。这种肽后依次跟有短间隔序列（Gly-Gly-Gly-Ser）和野生型 pIII 序列。该库包含约 10 亿个电穿孔序列，在提供的 10 µl 噬菌体中经扩增一次，每个序列即可产生约 100 个拷贝。

噬菌体展示文献引用

Ph.D.™-12 噬菌体展示肽库试剂盒 |

对 pIII 上展示的五价肽库进行淘选。

产品类别:
Discontinued (<3 years)

  • 相关产品

    相关产品

    • Ph.D.™ 多肽展示克隆系统

    单独销售的组分

    • Ph.D.™-12 噬菌体展示肽库
    • e4104-ecoli-k12-er2738

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操作说明、说明书 & 用法

  • 操作说明

    1. M13 Titer Protocol for Ph.D. Phage Display
    2. M13 Amplifcation Protocol for Ph.D. Phage Display

  • 说明书

    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE8100_E8101_E8110_E8111_E8120

  • 应用实例

    • Ph.D.™ Peptide Display Cloning System

FAQs & 问题解决指南

  • FAQs

    1. Which of the three ready-made libraries should I choose?
    2. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
    3. No plaques are visible when titering using the Ph.D.™ Phage Display kit.
    4. I am using Ph.D.™ Phage display and the amplified phage titer is low.
    5. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield a readable sequence.
    6. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
    7. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
    8. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
    9. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
    10. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
    11. Where can I find references for Ph.D.™ phage display libraries?

  • 问题解决指南

    • Phage Display Troubleshooting Guide