D/L-乳酸[D-/L-乳酸盐]检测试剂盒 D/L-Lactic Acid (D-/L-Lacate) Assay Kit 货号：K-DLATE Megazyme中文站

发表于由

The D-/L-Lactic Acid (D-/L-Lactate) (Rapid) test kit is used for the rapid and specific concurrent measurement and analysis of L-lactic acid (L-lactate) and D-lactic acid (D-lactate) in beverages, meat, dairy and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of D-/L-Lactic Acid in
foodstuffs, beverages and other materials

Principle:
(D-lactate dehydrogenase)
(1) D-Lactic acid + NAD+ ↔ pyruvate + NADH + H+

(L-lactate dehydrogenase)
(2) L-Lactic acid + NAD+ ↔ pyruvate + NADH + H+

(glutamate-pyruvate transaminase)
(3) Pyruvate + D-glutamate → D-alanine + 2-oxoglutarate

Kit size:                          50 assays of each
Method:                          Spectrophotometric at 340 nm
Reaction time:                ~ 10 min (L-lactic acid) and ~ 5 min (D-lactic acid)
Detection limit:               0.21 mg/L
Application examples: 
Wine, soft drinks, milk, dairy products, foods containing milk (e.g. dietetic
foods, bakery products, baby food, chocolate, sweets and ice-cream),
vinegar, fruit and vegetables, processed fruit and vegetables, meat
products, food additives, paper (and cardboard), cosmetics, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by DIN, GOST,
IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK

Advantages

  • Rapid total analysis time (concurrent / flexible D and L-lactic acid reaction format)
  • D-lactate dehydrogenase reaction very rapid with most samples (~ 5 min)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability