Ribonucleoside Vanadyl Complex |NEB酶试剂 New England Biolabs

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产品信息

Ribonucleoside-vanadyl complex is a potent inhibitor of various ribonucleases (1,2,3). This complex is compatible with cell fractionation methods as well as sucrose-gradient centrifugations. The 200 mM stock solution is reconstituted to a green-black clear solution by incubating the sealed vial at 65°C. Once open, the entire sample should be aliquoted into smaller samples and frozen.

The ribonucleoside-vanadyl complex should be added to all buffers to a final concentration of 10 mM. The buffers should not contain EDTA since one equivalent will totally dissociate the complex.

We do not recommend the use of the vanadyl complex in cell-free translation systems and with reverse transcriptase (5). The vanadyl complex can be used in the selective degradation of DNA while preserving RNA since pancreatic deoxyribonuclease I is not inhibited (5). Removal of the ribonucleoside-vanadyl complex from the RNA can be accomplished by adding 10 equivalents of EDTA before ethanol precipitation.

产品来源

This vanadyl complex is prepared from a modification of procedures by Lienhard using all four ribonucleosides (4).

产品类别:
RNase Inhibitors

  • 产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • S1402S     -20    
        Ribonucleoside Vanadyl Complex S1402SVIAL -20 1 x 10 ml 200 mM

  • 特性和用法

    货运单

    • Ships on dry ice

  • 注意事项

    1. Concentration of ribonucleoside-complex at 50% inhibition 2.4 mM.
    2. The toxicological properties of this compound have not been fully investigated
    3. Avoid contact with skin.

  • 参考文献

    1. Gray, J.C. (1974). Arch. Biochem. Biophys.. 163, 343-348.
    2. Egberts, E., Hackett, P.B. and Traub, P. (1971). Hoppe-Zeyler’s Z. Physiol Chem.. 358, 475-490.
    3. Leinhard, G.E., Secemski, I.I., Koehler, K.A. and Lindquist, R.N. (1971). Cold Spring Harbor Symp. Quant. Biol.. 36, 45-51.
    4. Berger, S.L., Hitchcock, N.J.M., Zoon, K.C., Birkenmier, C.S., Friedman, R.M. and Chang, E.H. (1980). J. Biol. Chem.. 21, 4602-4608.

FAQs & 问题解决指南

  • 实验技巧

    Please, make sure this product is stored frozen until just before using or aliquotting.

    This product is very susceptible to oxidation so it is recommended to aliquot the solution as needed and avoid refreezing any leftover solution.