产品信息

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  M0545S     -20       Exonuclease VIII, truncated M0545SVIAL -20 1 x 0.1 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

• 特性和用法

  单位定义

  One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [³H] DNA.

  反应条件

  1X NEBuffer™ 4
  Incubate at 37°C

  1X NEBuffer™ 4
  50 mM Potassium Acetate
  20 mM Tris-acetate
  10 mM Magnesium Acetate
  1 mM DTT
  (pH 7.9 @ 25°C)

  贮存溶液

  50 mM Tris-HCl
  100 mM NaCl
  0.1 mM EDTA
  1 mM DTT
  0.1% Triton® X-100
  50% Glycerol
  pH 7.5 @ 25°C

  热失活

  70°C for 15 minutes

  单位活性检测条件

  1X NEBuffer 4 with 0.15 mM with sonicated duplex ³H- DNA.

• 优势和特性

  应用特性

  •    Degradation of linear dsDNA while maintaining double stranded circular DNA.

• 参考文献

  1. Chang, et al. (2001). J. Biol. Chem.. 46004–46010.

操作说明、说明书 & 用法

• 操作说明

  1. Protocol for Exonuclease VIII, truncated (M0545)

工具 & 资源

• 选择指南

  • Activities of Exonucleases and Non-specific Endonucleases
  • Common Applications for Exonucleases and Endonucleases
  • Properties of Exonucleases and Non-specific Endonucleases

• Web 工具

  • Exo Selector

• 研究摘要

  • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

• FAQs

  1. How does Exonuclease VIII, truncated differ from Lambda Exonuclease (NEB# M0262)?

  2. Will Exonuclease VIII, truncated cleave supercoiled dsDNA or covalently close circular form dsDNA?

  3. How does Exonuclease VIII, truncated differ from Exonuclease III (NEB# M0206)?
  4. Can DNA be blunted using Exonuclease VIII, truncated?
  5. What is the activity of Exonuclease VIII, truncated in these common buffers?